FFPE-FASP Protein Digestion Kits
De-paraffinization and Uncrosslinking. Unbiased Extraction and Complete Solubilization. Same-As-Fresh Quantification. Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. Based on a spin-filter sample preparation method initially described by Manza, et al., and developed further and extended to FFPE tissue processing by Ostasiewicz, et al., this method features unbiased extraction, complete proteome solubilization, and highly efficient digestion. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome.