One-Step PolyMAC for homogeneous enrichment of phosphopeptides prior to mass spectrometry analysis
PolyMAC is based on a metal-ion functionalized soluble nanopolymer to chelate phosphopeptides in a homogeneous aqueous environment. Compared to current strategies based on solid phase micro- and nano-particles, PolyMAC demonstrates outstanding reproducibility, exceptional selectivity, fast chelation times, and high phosphopeptide recovery from complex mixtures.
Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. Available here is a novel chemical strategy termed Polymer-based Metal-ion Affinity Capture (PolyMAC) for the isolation of phosphopeptides with exceptionally high reproducibility, selectivity, and sensitivity.
One-step PolyMAC advantages
- Higher reproducibility between experiments and labs
- Single step phosphopeptide and PolyMAC capture
- Improved phosphopeptide recovery
- Exceptional selectivity
- Outstanding reproducibility
- Fast chelation times
The in-solution binding of phosphopeptides to PolyMAC and the covalent capture onto solid-phase beads all occur in a single step. At this point, we offer capture based on magnetic beads only.
All kits include PolyMAC-Ti with magnetic capture beads, loading buffer, two washing buffers and elution buffer.
A magnetic separator rack is not included.
In-solution phosphopeptide enrichment using PolyMAC-Ti
100ug of digested complex cell lysate from DG-75 B-cell lymphoma cells was used to compare the enrichment efficiency between titanium-based PolyMAC and other commercially available sources of phosphopeptide enrichment reagents. A number of unique phosphosites identified and percent enrichment selectivity were used as the parameters for comparison. Each experiment was run in triplicate.