Spin-Tip PolyMAC-Ti Phosphopeptide Enrichment Kit
Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful mass spectrometry-based phosphoproteomics experiments. PolyMAC offers an efficient and greatly improved method to achieve more complete phosphopeptide enrichment under homogeneous conditions. This highly selective enrichment procedure can be used with majority of the complex samples because it provides optimal specificity toward phosphopeptides.
The performance of the PolyMAC kit was evaluated using highly complex lysate samples from different sample origins. Each experiment was a single 60-min LC-MS analysis, and the number of unique phosphopeptides identified and percent enrichment selectivity are reported in the results.
|Sample||Number of unique phosphopeptides||Enrichment selectivity (%)|
|30 µg Human B cells lysate||8752||96|
|100 µg Arabidopsis thaliana lysate||5497||90|
|1 mg Rice fungus lysate||2974||91|
|48 Enrichments||96 Enrichments|