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Streptavidin Check&Go!

Streptavidin Check&Go! allows users to confirm the successful conjugation of streptavidin to their antibody, without the need for any specialized or costly equipment.

It is a quick and simple immunochromatography test that requires only 10 minutes and very small quantities of the streptavidin conjugated antibody (See Figure 1.).

The key components of the kit include a nitrocellulose membrane containing a “Test line” of immobilized Protein A and Protein G, both of which have a high affinity for the Fc region of a variety of IgG molecules, and  40nm InnovaCoat® GOLD Biotin for detection.

  • Quickly check the success of your conjugation (only 10 minutes!)
  • Easy to use
  • No costly equipment required
  • Compatible with antibody conjugates generated using our Lightning-Link® Streptavidin antibody labeling kits and other Streptavidin labeling technologies that are commercially available
  • Requires only small amounts of conjugated antibody (10 ul of diluted conjugate)
  • Unique to Expedeon

After performing your Streptavidin-IgG conjugation reaction, you can visually check the success of the conjugation by simply running your sample on the strip.

 

Figure 1. Schematic diagram of Streptavidin Check&Go! Kit

Once the streptavidin-antibody conjugate is mixed with the 40nm InnovaCoat® GOLD Biotin it forms a complex that flows along the nitrocellulose membrane. When this complex is bound by the Protein A and Protein G concentrated on the “Test line”, a visible red line appears on the strip that confirms that the conjugation was successful.

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Figure 1.  Rabbit IgG has been conjugated with Lightning-Link® Streptavidin and run on Protein A/G strips at different concentrations together with 0.2OD InnovaCoat® GOLD Biotin.  The signal intensity varies based on the antibody species.

Figure 2. Signal intensity of different Streptavidin-Antibody conjugates

 

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