Streptavidin Check&Go! allows users to confirm the successful conjugation of streptavidin to their antibody, without the need for any specialized or costly equipment.
It is a quick and simple immunochromatography test that requires only 10 minutes and very small quantities of the streptavidin conjugated antibody (See Figure 1.).
The key components of the kit include a nitrocellulose membrane containing a “Test line” of immobilized Protein A and Protein G, both of which have a high affinity for the Fc region of a variety of IgG molecules, and 40nm InnovaCoat® GOLD Biotin for detection.
- Quickly check the success of your conjugation (only 10 minutes!)
- Easy to use
- No costly equipment required
- Compatible with antibody conjugates generated using our Lightning-Link® Streptavidin antibody labeling kits and other Streptavidin labeling technologies that are commercially available
- Requires only small amounts of conjugated antibody (10 ul of diluted conjugate)
- Unique to Expedeon
After performing your Streptavidin-IgG conjugation reaction, you can visually check the success of the conjugation by simply running your sample on the strip.
Figure 1. Schematic diagram of Streptavidin Check&Go! Kit
Once the streptavidin-antibody conjugate is mixed with the 40nm InnovaCoat® GOLD Biotin it forms a complex that flows along the nitrocellulose membrane. When this complex is bound by the Protein A and Protein G concentrated on the “Test line”, a visible red line appears on the strip that confirms that the conjugation was successful.Click to read more