imm-Link™ immunogen preparation kits conjugate small antigens (haptens) to carrier proteins. An antigen is defined as any substance that induces an immune response resulting in the production of antibodies in the host species. The immune response is commonly used to generate antibodies (monoclonal and polyclonal) against proteins of interest for use in applications such as Western blotting, immunohistochemistry and flow cytometry.
Haptens tend to be poor at generating an immune response when used alone. In order to maximize a response, they are often covalently linked to larger “carrier” molecules such as Bovine Serum Albumin (BSA), Ovalbumin or Keyhole Limpet Hemocyanin (KLH).
The imm-Link™ immunogen preparation kits are quick and easy to use. Only 20–30 seconds hands-on time is typically required to set up the conjugation reaction, which is performed by simply adding the hapten solution to the lyophilized mixture containing the carrier protein and required conjugation chemistry.
|imm-Link™ Immunogen Kit||Target Functional Groups|
|Carboxyl||Glutamic acid, aspartic acid or free carboxyl groups|
|Sulfhydryl||Cysteines or free sulfhydryl groups (SH)|
|Amine||Lysines or free amines (NH2)|