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Lightning-Link® PerCP/Cy5.5

Lightning-Link® antibody labeling kit

The Expedeon Lightning-Link®  PerCP/Cy5.5 antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to PerCP/Cy5.5 with only 30 seconds hands-on time and with 100% recovery of materials. The technology is fully scalable from 10ug to 1g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.

Lightning-Link_process_diagram

PerCP/Cy5.5 is a tandem label. The PerCP excited at 484nm and functions as an energy donor for the Cy5.5. Energy is transferred from the PerCP to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PerCP in the form of long wavelength light at 692nm.

Please refer to our custom services for bulk orders or get in contact with us to know more about this product.

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Flow cytometry data Lightning-Link® PerCP/Cy5.5

Figure 1. Mouse anti-human CD3 was conjugated with PerCP/Cy5.5 using an Expedeon Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line – negative control; red line – positive staining).

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