Lightning-Link® Metal Labeling Kits

Current metal conjugation protocols can be complex and time consuming procedures, and may result in low antibody recovery. Lightning-Link Metal Labeling Kits allow metal / antibody labeling to be set up in less than 30 seconds – simply add the antibody solution to the activated metal ligand!

How Does It Work?

3_step_metal_labeling_image

 

Features and Benefits of Lightning-Link Metal Labeling Kits

  •  30 seconds hands-on time
  •  Complete labeling in only 1 hour
  •  Scalable labeling of your antibody – label as little as 10 μg or 100 μg at a time
  •  No separation steps, 100% antibody recovery
  •  Gentle chemistry, stable conjugates
  •  Range of metals available to enable multiplexing options
  •  Excellent batch-to-batch reproducibility
  •  Wide range of research applications

Applications

  • X-ray fluorescence
  • Mass cytometry
  • Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
  • Dissociation-enhanced fluorescence immunoassays (**DELFIA®)

**DELFIA® is a registered trademark of Perkin Elmer

DATA_Lightning-Link_Metal_Sandwich_ELISA

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Gel Image Showing Lightning-Link® Metal Labeling Kit Antibody Labeling Efficiency

DATA_Lightning-Link_Metal_Gelv2

 

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

 

DATA_Lightning-Link_Metal_XRFSpectra 2

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link® Metal Labeling kit.

 

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