Thunder-Link® PLUS Conjugation Kit
Thunder-Link® PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods.
Thunder-Link® PLUS Features and Benefits
- Quick and easy to use – Save time, no specialist knowledge required
- High levels of antibody and oligo recovery – Save precious reagents
- Use your own oligo and antibody, at your desired ratio – Flexible
- Freeze dried – Ships at ambient temperature, long shelf life
- Stringently QC tested – Consistent high quality, excellent batch to batch reproducibility
- Unidirectional chemistry – No risk of crosslinking
- Covalent bond – Highly stable conjugates
- Suitable for single stranded oligos of 10–120 bases, double stranded oligos up to 80 base pairs – Covers the majority of sequences
- Linking chemistry works at both 5’ and 3’ end – Provides ability to combine with other modifications
- Postconjugation clean up step – No interference from unbound oligo’s
- Positive control antibody and oligo included – Enables confirmation of protocol success
- A wide range of target proteins – Also applicable to antibody fragments and small proteins.
Main applications: immunoPCR, Proximity Ligation Assay, Electrochemical Proximity Assay, CITEseq and REAPseq.
Advantages of Thunder-Link® PLUS:
- Only 30 minutes antibody and oligo activation
- Fast oligo conjugation – Now only one hour!
- High antibody and oligo recovery
- Robust and flexible clean up procedure (now works with antibody fragments and other proteins).
Thunder-Link® PLUS Kit Components
- Thunder-Link® PLUS Oligo Activation reagent
- Thunder-Link® PLUS Antibody Activation Reagent
- Freeze dried control oligo
- Freeze dried control antibody
- Separating columns
- Thunder-Link® PLUS Wash buffer
- Thunder-Link® PLUS Conjugate Clean Up Reagent
- Thunder-Link® PLUS Antibody Suspension Buffer.