LifeXtend HRP Conjugate Stabilizer

The performance of HRP conjugates diminishes over time. There are a number of factors that contribute to loss of performance including:

  • Inactivation of HRP
  • Instability of the bond which links HRP to the antibody
  • Microbial attack
  • Denaturation of the antibody
  • Loss of performance accelerates with increasing temperature and with increasing dilution of the conjugates.

For these reasons HRP conjugates are often kept in a concentrated form at 4oC.

LifeXtendTM HRP conjugate stabilizer / diluent is a proprietary multicomponent reagent system that protects antibody–HRP conjugates from all of the negative factors noted above, thus ensuring the best possible performance in experiments performed at room temperature. Moreover, the ability to store HRP conjugates at working dilutions eliminates waste and improves consistency from experiment to experiment.

In addition to its proprietary enzyme stabilizers, LifeXtendTM contains antimicrobial agents as well as blockers to enhance signal to background in immunoassay applications.

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Figure 1. Comparison of LifeXtend™ with other well known brands of stabilizer. HRP conjugated goat antirabbit IgG (1mg / ml) was diluted 1 / 10,000 in each of four stabilizers and incubated for 12 days at 37oC. An undiluted control sample was incubated at 4oC for the same period and diluted 1 / 10,000 prior to ELISA testing. Conjugates were incubated on a rabbit IgG coated plate for one hour at room temperature, followed by washing and determination of HRP activity using ABTS substrate.

As can be seen above, even some of the best known stabilizers perform either poorly or only modestly in standard accelerated stability trials at 37oC. LifeXtendTM HRP stabilizer / diluent affords the greatest protection and can substantially extend the shelf life of HRP conjugates stored at 4oC or at room temperature.