Protein A–R-PE conjugate

Expedeon’s Protein A–R-Phycoerythrin conjugates are manufactured using our Lightning-Link® antibody labeling technology, to provide extremely high quality, stable conjugates. We are currently the only supplier of Protein A–R-PE conjugates. For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze dried for shipping at ambient temperature.

Specifications

SpecificityMost mammalian IgGs
Host speciesE.coli and Red Algae
FormatLyophilized
Product size100ug and 1mg total protein
BufferPhosphate Buffer
Storage-20°C

Protein A is a 42kDa surface protein of the bacterium Staphylococcus aureus, which binds the Fc region of many mammalian IgGs. Its high affinity for immunoglobulins is the reason that it is often used in biochemical research. 

R-Phycoerythrin (R-PE) is a 240kDa phycobiliprotein that is isolated from red algae. R-PE has a bright red-orange fluorescence with a high quantum yield, and is brighter than other conventional fluorophores and phycobiliproteins. The absorbance spectrum of R-PE covers a range of excitation wavelengths, which provides an advantage for multicolor immunofluorescent staining or cell sorting. The emission maximum of R-PE is at 575nm. R-PE is used for fluorescence based detection, often in assays that require high sensitivity.

Fluorescence Intensity of Expedeon’s Protein A–R-Phycoerythrin from Two Batches

Figure 1. Two batches of Protein A – R-Phycoerythrin conjugates made with Expedeon’s Lightning-Link® conjugation technology were tested in ELISA as shown. Both batches perform similarly.

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