The RunBlue™ prestained Molecular-Weight Markers for SDS PAGE contains 10 recombinant proteins ranging in size from 7.6 kDa to 195 kDa. For easy visualization the 28 kDa and 71 kDa marker have been dyed orange, the other eight markers are blue. The purity and band sharpness mean the markers can be used for visualizing the separation while running the gel, checking transfer effectiveness of your western blot, or as a routine marker in place of other unstained markers.
Recommend Load Volumes
RunBlue™ prestained markers are provided ready to use in 1x RunBlue™ sample buffer. Recommended volumes to load are:
|Application||12 well volume||12 well loads/vial||17 well volume||17 well loads/vial|
|Monitor during Run||10 μl||60||5 μl||120|
|Visible after blotting||4 μl||150||2 μl||300|
|Visible after staining||4 μl||150||2 μl||300|