RunBlue™ Native Protein Gels

RunBlue™ Performance

RunBlue™ Native Protein Gels take advantage of Expedeon’s new methods and innovations to the gel making process to increase resolution and reproducibility of separated protein bands. They allow separation across a range of molecular weights and a constant pH throughout the gel prevents changes in protein mobility as the gel runs. RunBlue™ Protein Gel’s proprietary polymerisation process results in more uniform gels between batches, with decreased variability and improved repeatability of results.  Our RunBlue™ Native Protein Gels are also available as mini 8x10cm sizes.

Key Benefits of RunBlue™ Native Protein Gels:

  • Optimised gel-buffer system– High resolution and sharp bands.
  • Extra resolving length – Increased separation.
  • Unique homogeneous polymerisation – Increased consistency and no residual free acrylamide.
  • Extra Wells – Run up to 12 or 17 samples simultaneously.
  • Deep and wide wells – Load up to 35 μl (12 well) or 20 μl (17 well).
  • Standard format – Compatible with 10x10cm protein gel tanks such as NuPAGE® XCell SureLock™ Mini-Cell.
  • Protruding teeth – No well contamination in case of overloading.
  • Resilient gel matrix – Tear-proof gel and risk-free handling.
  • Comb-free – No potentially broken or damaged teeth.
  • Strip-free – No potentially ruined Western Blots.
  • Clipped cassette – Fast and gentle opening.
  • Neutral buffer and high chemical stability – shelf life of 2 years at 4ºC and 6 months at room temperature.

Usability Enhancements

In addition to their high resolution separation of protein all RunBlue™ Protein Gels have a number of enhancements to improve and streamline usability. RunBlue™ Protein Gels are up to 10 times stronger than conventional hand-cast gels and can stand up to repeated handling. This practically eliminates the risk of torn and unusable gels encountered during normal gel manipulation. More lanes than standard gels allows more proteins to be run and wide, deep wells allow higher volumes to be loaded.

Cassettes have no comb or tape to remove and no excess gel to trim, all of which improves the speed and efficiency of the loading and running process. Wells are outlined and numbered for quicker sample loading and the cassette is labelled with gel type, percentage and a unique lot number for traceability. Well teeth protrude above the cassette to prevent sample contamination between wells in case of overloading.

Flexible Usage

RunBlue™ Native Protein Gels are highly optimised for a Tris-Tricine buffer system and must be run with this buffer system to achieve good results. Runblue™ Native Run Buffer is available as a premixed 20X solution, or a homemade version can be mixed using the available recipe. The samples must be prepared with the Tris-Tricine based RunBlue™ Native Sample Buffer which is available as a premade 4X solution, or can be homemade with the available recipe. RunBlue™ Protein Gels have an extended shelf life of 2 years at 4ºC which is ideal for large stocks or infrequent use. They also have a 6 month shelf life at room temperature which is convenient when fridge space is limited. The RunBlue™ SDS Protein Gel 10x10cm cassettes are designed to fit a number of gel tanks such as Invitrogen® XCell SureLock™ equipment, Hoeffer, and Owl tanks.

Improved Safety

Polyacrylamide gels are based on the polymerisation of liquid acrylamide and bis-acrylamide. The polymerisation is usually induced by the addition of a source of free radicals and a stabilizer (usually APS & TEMED) which induces cross link formation between acrylamide and bis-acrylamide monomers. This localised chemical induction results in heterogeneous polymerisation across the gel due to acrylamide concentration gradients being generated, and can lead to the presence of residual free acrylamide at the end of the polymerisation.

Expedeon has developed a unique physical induction technology which ensure homogenous polymerization across the whole gel and no residual, acrylamide free after the end of the polymerization. Numerous studies have shown the acrylamide monomer is a neurotoxin and possible carcinogen*. Acrylamide can be inhaled as a dry powder, evaporate from liquid solution and sublimate from solid gels if not fully polymerised. RunBlue™ gels are polymerised in a unique homogenous manner that results in a consistent gel with minimal or no residue after casting.

* AIHA J (Fairfax, Va). 2002 Jul-Aug;63(4):468-73. Inhalation exposures to acrylamide in biomedical laboratories.

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