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Ponceau S solution

Ponceau S solution

Ponceau S solution is a staining reagent for the detection of proteins on nitrocellulose and PVDF membranes. Ponceau S solution allows users to check the efficiency of protein transfer to blotting membranes during Western blotting before membrane blocking and protein detection.

Benefits and features:

  • Ready to use solution
  • Results within 5 minutes.

Users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution following protein transfer. The transferred protein becomes rapidly visible as red bands on the membrane, with the highest intensity of staining reached within 5 minutes of  incubation with Ponceau S solution. Rinsing the membrane with water after Ponceau S staining can help reduce ‘background’ staining on the blotting membrane. The intensity of staining corresponds to the amount of protein transferred.

Protein transfer using Ponceau S solution

Kit Contents:

1L reagent, containing Ponceau S and acetic acid in water

Please refer to our custom services for bulk orders or get in contact with us to know more about this product.

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Figure 1: Runblue 4-12% Bis-Tris gel. Lanes 1,8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2ug BSA, lane 5 1ug BSA, lane 6 500ng BSA, lane 7 250ng BSA.

Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce ‘background’ staining on the blotting membrane. The intensity of staining corresponds to the amount of protein transferred.

 

Is Ponceau S solution suitable for both nitrocellulose and PVDF membranes?

Yes, it is. Ponceau S solution has been tested on both nitrocellulose and PVDF membranes and it provided reproducible data as well as a high signal-to-noise ratio.

Is Ponceau S solution suitable with different blocking agents other than BSA?

Yes, it is. Ponceau S solution is suitable with the most common-use blocking agents, such as non-fat dry milk (3-5%), casein (1%) and fish skin gelatin (1%).

What is the recommended dilution for Ponceau S solution ?

Ponceau S solution is provided as a ready-to-use 1X solution

Can Ponceau S solution be used to detect proteins under both reducing and non-reducing conditions?

Yes, it can. Ponceau S solution has been tested on proteins run under both reducing and non-reducing conditions, with both conditions showing equal sensitivity of detection and reproducibility.

What is the limit of detection for Ponceau S solution?

Ponceau S solution can stain as little as 250ng of Bovine Serum Albumin on a nitrocellulose membrane.

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