Amintra® Heparin Affinity Resin
is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
The base matrix is made of cross-linked porous agarose. The particles have an open pore structure with good mass transfer properties to large protein molecules. Heparin is immobilized to the base matrix by reducing amination method.
The medium shows high mechanical rigidity, so it can be operated at high flow velocities with moderate pressure drops. The medium is compatible with most of the chemical reagents commonly used in biological systems.
|Functional group||Heparin of porcine origin|
|Ligand density||4 - 5 mg/ml|
|Particle size||50 - 150 µm|
|pH stability||4-13 (short term) and 4-12 (long term)|
|Working temperature||+4OC to +30OC |
|Chemical stability||Compatible with commonly used buffers,|
6M guanidine-HCl, 8 M urea
|Storage||0.05 M sodium acetate in 20% denatured|