Amintra® Nickel Magnetic Affinity Resin
Increasingly, Amintra® Ni-NTA and Ni-IDA resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells.
Typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 μl of resin is required for protein purification, it is difficult to handle such small quantities in columns. Amintra® Nickel Magnetic resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).
- Tailored base matrix design for batch magnetic purification of proteins
- High binding capacity (could be over 40 mg/ml)
- Simple and convenient operation, no chromatography training required
- Flexible operational conditions
- Low cost
Protein Purification Procedure