Amintra® NHS activated is a bead formed, highly crosslinked preactivated matrix prepared by coupling Amintra® NHS activated with 6-aminohexanoic acid via a spacer arm, specifically designed for affinity purification. The terminal carboxyl group is activated by esterification with N-hydroxysuccinimide. Ligands containing primary amino groups couple directly to this active ester to form a chemically very stable amide linkage. To maintain high activity, Amintra® NHS activated is supplied as a suspension in 100% isopropanol, which should be washed away before use. Specific regulations may apply when using this product since it can require the use of explosion proof areas and equipment.
|Mean particle size||90μm|
|Particle size range||45 to 165μm|
|Bead structure||Highly cross linked 4% agarose, spherical|
|Linear flow velocity 1||150cm / h at 100kPa|
|Ligand density||16 to 23μmol NHS / ml drained medium|
|pH stability 2||long term: 3 to 13; short term (CIP): 2 to 13|
- At 25°C, 25cm bed height (base matrix). The flow velocity after coupling may differ depending on the ligand
- Depends on the ligand. Tested with lysine as ligand with single point attachment.