Immunochromatography Test for His-tagged Proteins in less than 15 Minutes
The His-Tag Check&Go! kit is a quick and easy competitive immunochromatography test that has been developed to allow scientists to verify and monitor successful expression of His-tagged recombinant proteins in one easy step, prior to performing other downstream applications.
Unlike traditional methods of assessing the success of your protein expression and purification, which can be tedious and time-consuming, His-Tag Check&Go! is a simple qualitative immunochromatography test that requires only 10–15 minutes of your time and very small quantities of your sample.
The key component of the kit is a nitrocellulose membrane containing an immobilized His-Tag protein on the ‘Test line’ (T line) and an ULFA-tagged protein on the ‘Control line’ (C line). A 40nm InnovaCoat®-GOLD antiHis-Tag antibody and 40nm InnovaCoat®-GOLD antiULFA tag conjugate are used for detection.
Why Choose Check&Go! for Qualitative Immunochromatography Tests?
• Quick yes / no answer during expression and purification processes
• Results in less than 15 minutes!
• Compatible with cell culture media and lysate
• Easy to use
• Cheaper than gel electrophoresis / Western Blot
• Small sample volume required: 80ul diluted* sample / strip
• Stable C line signal intensity: Independent from his-tagged protein levels
• Compatible with Amintra™ CoHis, NiHis and Ni-NTA resins
• Unique to Expedeon
* Subject to protein yield
If your sample does not contain any His-tagged protein, the T line will appear as a strong red line (Figure A: -His-tagged protein).
If your sample does contain His-tagged protein, the T line will not be visible (or it will appear as a pale red line) as it competes with the binding of the InnovaCoat® -GOLD anti-His-Tag antibody conjugate to the antigen immobilized on the T line (Figure B: +His-tagged protein).
The C line is assay independent and should always appear as a strong red line – if it is not visible, the test is not valid and should be repeated.
|1X PBS||Fully compatible|
|1X TBS||Fully compatible|
|RIPA buffer||Fully compatible|