Amintra™ MBP resin is a chromatography medium for purifying recombinant proteins tagged with maltose binding protein (MBP). MBP tagging of recombinant proteins can result in increased expression levels and higher solubility of a target protein, while allowing high purity recovery with a one step purification.
Tagging proteins with MBP often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. With the benefit of increased solubility, the tag could be particularly useful for recombinant proteins expressed in an insoluble form (inclusion bodies).
Affinity purification using Amintra MBP resin takes place under physiological conditions and mild elution is performed using maltose. This preserves the activity of the target protein. Even intact protein complexes may be purified. In addition, the high specificity of the binding means that very high purity can be achieved in just one step in combination with high binding capacity. The medium shows high mechanical rigidity, so can be operated at high flow velocities with moderate pressure drop.
Amintra MBP resin is a robust, high resolution medium based on the well-known agarose matrix. The small and evenly sized beads ensure that MBP-tagged proteins elute in narrow peaks, minimizing the need for further time consuming concentration steps. Amintra MBP resin tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same medium to be used for repeated purifications.
|Matrix||Highly cross-linked agarose|
|Functional group||Polysaccharide containing maltose|
|Particle size||30 – 50 mm|
|Binding capacity||Protein dependent|
|pH stability||2-13 (short term) and >7 (working range)|
|Working temperature||+4°C to +30°C|
|Chemical stability||All commonly used buffers 6M guanidine-HCl 8 M urea 0.5 M NaOH (for regeneration and cleaning)|
|Storage||Store in 20% denatured ethanol|