Amintra® NHS activated

Amintra® NHS activated is a bead formed, highly crosslinked preactivated matrix prepared by coupling Amintra® NHS activated with 6-aminohexanoic acid via a spacer arm, specifically designed for affinity purification. The terminal carboxyl group is activated by esterification with N-hydroxysuccinimide. Ligands containing primary amino groups couple directly to this active ester to form a chemically very stable amide linkage. To maintain high activity, Amintra® NHS activated is supplied as a suspension in 100% isopropanol, which should be washed away before use. Specific regulations may apply when using this product since it can require the use of explosion proof areas and equipment.

Discover more about affinity purification with our downloadable guide

Characteristics

Mean particle size90μm
Particle size range45 to 165μm
Bead structureHighly cross linked 4% agarose, spherical
Linear flow velocity 1150cm / h at 100kPa
Ligand density16 to 23μmol NHS / ml drained medium
pH stability 2long term: 3 to 13; short term (CIP): 2 to 13

  1. At 25°C, 25cm bed height (base matrix). The flow velocity after coupling may differ depending on the ligand
  2. Depends on the ligand. Tested with lysine as ligand with single point attachment.

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