Amintra™ NHS-activated is a bead-formed, highly cross-linked pre-activated matrix prepared by coupling Amintra™ NHS-activated with 6-aminohexanoic acid via a spacer arm, specifically designed for affinity purification. The terminal carboxyl group is activated by esterification with N-hydroxysuccinimide. Ligands containing primary amino groups couple directly to this active ester to form a chemically very stable amide linkage. To maintain high activity, Amintra™ NHS-activated is supplied as a suspension in 100% isopropanol, which should be washed away before use. Specific regulations may apply when using this product since it can require the use of explosion-proof areas and equipment.
|Mean particle size||90 μm|
|Particle size range||45 to 165 μm|
|Bead structure||Highly cross-linked 4% agarose, spherical|
|Linear flow velocity 1||150 cm/h at 100 kPa|
|Ligand density||16 to 23 μmol NHS/ml drained medium|
|pH stability 2||long term: 3 to 13; short term (CIP): 2 to 13|
1 At 25°C, 25 cm bed height (base matrix). The flow velocity after coupling may differ depending on the ligand.
2 Depends on the ligand. Tested with lysine as ligand with single-point attachment.