Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions. The AbPure™ Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.
The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favourable conjugation buffer.
- Easy and quick
- High recoveries
- Specifically developed for our InnovaCoat® GOLD Nanoparticles and Conjugation Kits.
- Three spin cartridge / collecting tube assemblies (of up to 0.5ml)
- One bottle of AbPure™ Conjugation Buffer Conjugation buffer.
The antibody will be retained by the filter, while the buffer can be exchanged by centrifugation into a more suitable one. It is advisable to have at least 50ug of antibody in a volume of 100ul for the concentration step to be successful.
Please note that if other proteins (e.g. BSA, Gelatin) are present, they will be retained by the filter as well and concentrated.
For more information, please read our Antibody Purification Guide.
Please get in contact with us to know more about this product.