Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect™ Serum antibody purification system is a fast and simple method to purify antibodies from these types of media.
Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. AbSelect™ G Serum resin is prepared by coupling purified Protein G to agarose beads. It can therefore be used to purify IgG fractions from both serum and ascites fluid. The antibody is captured on the AbSelect™ G resin and unwanted substances are removed by a simple wash procedure. The antibody is then eluted and neutralized.
The components of the AbSelect™ G Serum antibody purification system are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
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