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Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.
Common commercial BSA removal techniques can involve many laborious steps. The Abselect™ BSA Removal Kit is a simple 1 step, 10 minute method which effectively separates the BSA from the antibody and can be used on any antibody sub-type or species.
Benefits of the AbSelect™ BSA removal kit:
- Only takes 10 minutes!
- Centrifuge is the only equipment required
- Also use to concentrate and exchange your buffer
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For more information, please read our Antibody Purification Guide.
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Q1. How much BSA can the BSA Removal Kit remove?
This 1ml kit can remove all of the BSA from up to 1.25ml of antibody, with a BSA concentration of 0.5% or less. For higher BSA concentrations, the method may need to be repeated, or a higher volume of BSA Removal Buffer may be required. Larger kit sizes could be provided if this is the case (see Q2).
Q2. Are larger pack sizes available?
Yes, specific pack sizes are available on request. Please contact our Technical Support Team for advice on specific pack sizes.
Q3. Can the BSA Removal Kit remove gelatin from my sample?
No, the kit is specifically designed for the removal of BSA. It is effective on some other buffers (see Q4), but is not effective on gelatin.
Q4. Could I use the BSA Removal Kit to remove Tris or Glycine from my antibody?
Yes, the kit will effectively separate the antibody in this situation. See section 5: Buffer composition for more information on suitable buffers for use with the kit.
Q5. Can the kit be used to purify antibody from TCS or serum?
No, the kit is not specific enough to the antibody to be used as a purification technique in this instance.
Q6. Can the BSA Removal Kit be used to concentrate a sample?
Yes, once the separation is complete, the antibody pellet can be recovered using any volume, to reach the desired final concentration.
Q7. Could the kit have any negative impact on the conjugation efficiency?
No, the BSA Removal Buffer has no effect on antibody conjugation using Lightning-Link® and Lightning-Link® Rapid kits.
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