Protection from Endogenous Proteases During Protein Extraction
The process of breaking open cell walls and subcellular compartments during protein extraction liberates endogenous proteases. Without effective inhibition, these enzymes will quickly diminish yields of intact proteins from cell and tissue extracts. The addition of protease inhibitors to lysis buffers used for protein extractions is an effective means of protecting extracted proteins from enzymatic degradation by endogenous proteases.
Proteoloc Protease Inhibitor Cocktails from Expedeon
Expedeon offers two protease inhibitor preparations for use with protein extraction lysis buffers: Proteoloc Protease Inhibitor Cocktail, and Proteoloc Protease Inhibitor Cocktail EDTA-free. Both products offer protection against loss of intact proteins due to endogenous protease activity. Proteoloc Protease Inhibitor Cocktails are provided as 100X stock solutions in DMSO.
Use the EDTA-free product if the next step in your workflow is isoelectric focusing, metal-affinity column separation, or another technique that is incompatible with chelation. Otherwise, use the standard Proteoloc Proteome Inhibitor Cocktail, which contains EDTA.
Proteoloc Inhibitor Cocktail is the protease inhibitor mixture recommended for use with Protein Discovery’s UPX Universal Protein Extraction and YPX Yeast Protein Extraction Kits.Click to read more