RunBlue™ TGS Blot Buffer has been specially formulated to provide optimal blot transfer of proteins from RunBlue™ Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue™ Dual Run and Blot system or a factor of 20 for use with other blotting methods.
RunBlue™ LDS Sample buffer (NXB31010) has been specifically formulated for use with RunBlue™ gels. The ions in the sample buffer match the gel buffer and the buffer has a higher density, making it compatible with the density of the running buffer. The buffer contains two tracking dyes. The Red dye is designed to monitor the pH of the buffer. A colour change from red to yellow indicates a shift in pH indicating that the buffer was poorly made or recycled too many times.
RunBlue™ SDS Running Buffer (NXB50500) a non-Reducing Tris-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can also be used for reducing separation by adding antioxidant (NXA30010) to the buffer prior to use.
RunBlue™ RAPID Running Buffer is a reducing, Tris-MOPS buffer system which provides a separation profile similar to MES buffer used with BIS-TRIS gels.
Simply choose which buffer will provide the greatest resolution for your protein(s) of interest.
Take a look at our Sample Buffers and Membranes for Western blotClick to read more