LumiBlue™ ECL Express Solution for Western Blotting
LumiBlue™ ECL Express is a solution with a standard level of signal intensity and is most effective for routine and housekeeping western blotting applications. The solution is sensitive enough to detect approximately 20pg of protein per band.
Signal intensity remains high for up to 16 hours after blotting and the working solution is stable for up to five days after activation, allowing convenient experiments across a working week and minimizing waste.
This kit provides a comparable level of sensitivity to Pierce™ ECL (Thermo Fisher Scientific) and Amersham™ ECL (GE Healthcare) with the same antibody dilutions, and does not require protocol reoptimization or changes from these kits.
Benefits of LumiBlue™ ECL Express include:
- Linear response – Novel acylation catalyst provide stable signal over a wide range of protein concentrations
- High sensitivity – Detect protein levels as low as 20pg per band with next generation enhancers
- Stable solution – Working solutions stable for up tofive days
- Long lasting signal – Perform multiple exposures with less signal loss
- Low background – Optimized formulation for lower backgrounds
- Bright bands – Equal or better brightness compared to other ECL solutions
- Standard protocols – Direct replacement of other ECL solutions with no protocol reoptimization or changes
- Long shelf life – One year shelf life at 4°C, convenient for infrequent Western blotters.
ECL kits are based on the enzymatic oxidation of luminol with peroxide, which produces light, usually through horseradish peroxidase (HRP). This method is employed by most traditional ECL kits. However, LumiBlue™ improves upon standard ECL kits by using a patented next generation electron mediator coupled with a novel acylation catalyst to improve performance (called enhancers).
The combination of these unique modifications to the ECL process increases reaction efficiency resulting in increased light intensity and less signal decay. Different LumiBlue™ kits are extensively optimized for specific antibody dilutions and protein levels giving higher intensity, longer signal stability and wider linearity over traditional ECL kits. This allows for more flexible and cost effective experimental design.
Five Day Working Solution
LumiBlue™ ECL solutions are activated by mixing the provided solutions in a 1:1 ratio. This allows smaller volumes to be prepared with LumiBlue™ than with other solutions that require asymmetric ratios such as 40:1. Convenient volumes can be prepared quickly and easily. Once mixed the LumiBlue™ ECL Express working solution is stable for up to five days, permitting larger batches to be made and used for a number of experiments across multiple days, saving time and ensuring that exactly the same solution is used for each blot.
*Antibodies diluted from 1mg / ml stock solution.
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