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Nucleic Acid Digestion

BaseMuncher is a non-specific endonuclease that hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length. Recombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is completely free of protease activity.  BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation. 

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
25,000 / 100,000 Units
Starting at: $279
Site Specific Proteases

Expedeon offer a Prescission site protease and a TEV protease. 3C-Express is a recombinant Human rhinovirus 3C protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
500 Units in 250 ul
$268.00
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5000 Units in 2500 ul
$2,112.00
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1000 Units in 50 ul
$182.00
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Protease Inhibitors

Expedeon protease inhibitor cocktails offer protection against loss of intact proteins due to endogenous protease activity. Proteoloc Protease Inhibitor Cocktails are available with or without EDTA and are provided as 100X stock solutions in DMSO.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
3x 10ml (100X)
$830.00
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3x 10ml (100X)
$830.00
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3ml (100X)
$180.00
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Protein Extraction & Solubilisation

The UPX and YPX kits are for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, or unbiased yeast protein extraction respectively. Both kits provide an abundance of starting material and do not require mechanical disruption. PPS Silent Surfactant is a cleavable surfactant capable of disrupting cell membranes and solubilizing proteins. At low pH, PPS hydrolyzes rapidly into cleavage products with no surfactant properties and no detergent interferences.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
10mg
$351.00
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5 x 1mg
$217.00
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Kit
$55.00
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MS Trypsin Digestion

FFPE protein digestion kits use aggressive, whole proteome solubilisation protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Both extraction and digestion occur efficiently using the same protocol. FFPE kits are available with or without filter-aided sample preparation steps.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
Kit
$71.00
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Kit
$71.00
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Phosphopeptide Isolation

Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. PolyMAC (Polymer-based Metal ion Affinity Capture) overcomes the shortcomings of current enrichment methods that typically lack the required reproducibility, phosphopeptide recovery and selectivity for high quality publishable data.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
25 / 50 / 100 reactions
Starting at: $217