Immunochromatography Test for GST-Tagged Protein Expression in Less Than 15 Minutes
The GST-Tag Check&Go! kit is a quick and easy sandwich lateral flow test that has been developed to allow scientists to verify and monitor successful expression of GST-tagged recombinant proteins in one easy step, prior to performing other downstream applications.
Unlike traditional methods of assessing the success of your protein expression and purification, which can be tedious and time consuming, GST-Tag Check&Go! is a simple qualitative immunochromatography test that requires only 10–15 minutes of your time and very small quantities of your sample.
The key component of the kit is a nitrocellulose membrane with a conjugate pad containing a mixture of anti-GST antibody, conjugated to either 40nm gold nanoparticles or CaptSure™ peptide whilst the “Test line” (T line) on the nitrocellulose contains an antibody which binds the CaptSure™ peptide. The intensity of the T-line correlates with the amount of GST-tagged protein in the sample.
The conjugate pads also contain biotin-gold, which binds streptavidin on the “Control Line” (C line) of the nitrocellulose strips. Hence, the C line is assay-independent and should always appear as a strong red line.
Each kit contains a vial of freeze-dried GST-tagged protein, which should be used as a positive control for the assay.
Why Choose Check&Go! for Qualitative Immunochromatography Tests?
- Quick yes / no answer during expression and purification processes
- Results in less than 15 minutes!
- Compatible with cell culture media and lysate
- Easy to use
- Cheaper than gel electrophoresis / Western blot
- Small sample volume required: 80ul diluted* sample / strip
- Stable ‘C line’ signal intensity: Independent from GST-Tagged protein levels
- Compatible with Amintra® Glutathione Affinity Resin and Amintra® Glutathione Magnetic Resin
* Subject to protein yield
Easy Visualization of Results for GST-Tag Check&Go! Expression Validation Kit:
If the sample does contain GST-Tagged protein, a strong T line will be visible as it interacts with the mixture of the InnovaCoat®-GOLD anti-GST Tag antibody conjugate and CaptSure™ peptide anti-GST Tag antibody conjugate, forming a ‘sandwich’, which can then bind to the antibody immobilized on the T line (see above: A: +GST tagged protein)
If the sample does not contain any GST-Tagged protein, the T line will not be visible or may appear as a weak line (see above: B: -GST tagged protein)
The C line is assay independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.
Assay compatibility and interfering substances:
|1X PBS||Fully compatible|
|1X TBS||Fully compatible|
|RIPA buffer||Fully compatible|
|Guanidine HCl||< 1mM|