Purified ctDNA Amplification Service
Workflow & Technical Information
TrueAdvance workflow for Purified ctDNA Amplification includes:
CovCheck Genome Coverage QC
CovCheck is our unique quality control assay for amplified human DNA samples. It is a PCR panel of 24 primers that can quickly determine genome coverage after amplification to screen samples suitable or not for further analysis. This prevents wasting your time and money on sequencing and data analysis. Our QC also includes quantification after amplification and DNA purification. We consider that a sample has been amplified successfully if the estimated genome coverage with CovCheck is 85% or more. You will receive a report of our QC for all submitted samples, and can decide if some samples that did not pass our CovCheck genome coverage QC can be considered successful anyway depending on the nature of your project.
Typical whole genome amplification QC is done by quantification of yield. This kind of QC is limited and only covers these potential issues, which are the less likely to happen:
• No cell / DNA in the tube / well
• Faulty WGA kit
• Sample buffer incompatibility with WGA kit.
Our unique CovCheck WGA coverage QC covers these issues, which are more likely to happen, especially with single cells:
• Damaged nucleus / cells
• Preferential binding of the primers to specific regions of the genome
• Nonspecific amplification artifacts
• Internal or external contaminations
• Bad DNA quality / quantity.
Pricing: You will only be charged for successful amplification results
Please fill in the form to the right to request more information on our Purified ctDNA Amplification service (up to 90 samples), or to get a quote for larger projects. Shipment of the samples to us is at your discretion. Samples will need to be shipped on blue ice. Shipment of the amplified samples back to you is included in our service prices.
For each plate, three negative controls (no template) three positive controls (human genomic DNA) are used.
Please Note: Samples need to be sent in PCR tubes, PCR strips or 96 well plates compatible with Bio-Rad PCR machines. For plates, six wells need to be empty for the controls.
The pricing structure for up to 90 samples follows:
|Number of Samples||Price of First Sample||Price per Extra Sample|
Our prices are disclosed in more details the dropdown box “Number of Samples”. “Price of First Sample” is the fixed minimum price charged for any services order and include the first sample passing our CovCheck genome coverage QC. “Price per Extra Samples” is the additional price per any samples passing our CovCheck genome coverage QC. And “Price for all Samples” it the total price if all samples passed our CovCheck genome coverage QC.
We do not charge for samples that do not pass our CovCheck genome coverage QC, and will be taken out of the total price when invoiced.