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Single Cell Whole Genome Amplification Service

Workflow and Technical Information

The TrueAdvance workflow for Single-Cell Whole Genome Amplification consists of:

 

 

 

 

For each plate, 3 negative controls (no template) and 3 positive controls (human genomic DNA) are used.

Note: Samples need to be sent in PCR tubes, PCR strips or 96 well plates compatible with Bio-Rad PCR machines. For plates, 6 wells need to be empty for the controls.

CovCheck Genome Coverage QC

CovCheck is our unique quality control assay for amplified human DNA samples. It is a PCR panel of 24 primers which can quickly determine genome coverage after amplification to screen samples suitable or not for further analysis. This prevents wasting your time and money on sequencing and data analysis. Our QC also includes quantification after amplification and DNA purification. We consider that a sample has been amplified successfully if the estimated genome coverage with CovCheck is 85% or more. Customers will receive a report of our QC for all submitted samples, and can decide if some samples that did not pass our CovCheck genome coverage QC can be considered successful anyway depending on the nature of your project.

Typical whole genome amplification QC are done by quantification of yield. This kind of QC are limited and only covers these potential issues, which are the less likely to happen:

  • No cell/DNA in the tube/well
  • Faulty WGA kit
  • Sample buffer incompatibility with WGA kit

Our unique CovCheck WGA coverage QC covers these issues, which are more likely to happen, especially with single-cells:

  • Damaged nucleus/cells
  • Preferential binding of the primers to specific regions of the genome
  • Nonspecific amplification artefacts
  • Internal or external contaminations
  • Bad DNA quality/quantity

Pricing : Customers will only be charged for for Successful Amplification Results.

To register your interest or for more information regarding our Single-Cell Whole Genome Amplification service, please get in touch using the the form to the right. Shipment of the samples to us is at your discretion. Samples will need to be shipped on dry ice. Shipment of the amplified samples back to you is included in our service prices.

The pricing structure for up to 90 samples follows:

Number of Samples Price of First Sample Price per Extra Sample.

Our prices are disclosed in more detail and can be found in the dropdown box which is listed as “Number of Samples”. The “Price of First Sample” is the fixed minimum price charged for any services order and includes the first sample passing our CovCheck genome coverage QC. “Price per Extra Samples” is the additional price per any samples passing our CovCheck genome coverage QC. and “Price for all Samples” is the total price if all samples passed our CovCheck genome coverage QC.

We do not charge for samples that do not pass our CovCheck genome coverage QC, and these will be taken out of the total price when invoiced.

Enquire below: