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Plasma ctDNA Amplification Service

Workflow and Technical Information

The TrueAdvance workflow for Plasma ctDNA Amplification consists of:

For each plate, three negative controls (no template) and three positive controls (human genomic DNA) are used.

Note: Samples need to be sent in PCR tubes, PCR strips or 96 well plates compatible with Bio-Rad PCR machines. For plates, six wells need to be empty for the controls.

CovCheck Genome Coverage QC

CovCheck is our unique quality control assay for amplified human DNA samples. It is a PCR panel of 24 primers that can quickly determine genome coverage after amplification to screen samples suitable or not for further analysis. This prevents wasting your valuable time and money on sequencing and data analysis. Our QC also includes quantification after amplification and after purification. We consider that a sample has been amplified successfully if the estimated genome coverage with CovCheck is 85% or more. You will receive a report of our QC for all submitted samples, and can decide if some samples that did not pass our CovCheck genome coverage QC can be considered successful anyway depending on the nature of your project.

Typical whole genome amplification QC is performed by quantification of yield. This kind of QC is limited and only covers the following potential issues, which are least likely to happen:

• No cell / DNA in the tube / well
• Faulty WGA kit
• Sample buffer incompatibility with WGA kit.

Our unique CovCheck WGA coverage QC covers these issues, which are more likely to happen, especially with single cells:

• Damaged nucleus / cells
• Preferential binding of the primers to specific regions of the genome
• Nonspecific amplification artifacts
• Internal or external contamination
• Bad DNA quality / quantity.

Pricing: We only charge for successful amplification results

Please fill in the form to the right to order our Plasma ctDNA Amplification Service, up to 90 samples, or to receive a quote for larger projects. Shipment of the samples to us is at your discretion. Samples will need to be shipped on dry ice. Shipment of the amplified samples back to you is included in our service prices.

The pricing structure for up to 90 samples follows:

Number of Samples Price of First Sample Price per Extra Sample
6 £818.18 £250.00
7–12 £1090.91 £200.00
13–24 £1636.36 £152.73
25–48 £2727.27 £109.09
49–90 £5000.00 £61.82

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